Total flavones of Desmodium styracifolium antagonize calcium oxalate monohydrate-triggered IL-2Rβ expression in renal epithelial cells

Increased calcium oxalate monohydrate crystal binding to injured renal tubular epithelial cells in culture.

The retention of crystals in the kidney is considered to be a crucial step in the development of a renal stone. This study demonstrates the time-dependent alterations in the extent of calcium oxalate (CaOx) monohydrate (COM) crystal binding to Madin-Darby canine kidney (MDCK) cells during their growth to confluence and during the healing of wounds made in confluent monolayers. As determined by ...

Mechanism of cytotoxicity of micron/nano calcium oxalate monohydrate and dihydrate crystals on renal epithelial cells

Calcium oxalate monohydrate crystals stimulate monocyte chemoattractant protein-1 and transforming growth factor β1 expression in human renal epithelial cells.

Crystal-cell interactions play a key role in the formation of kidney stones. Few studies have referred to the role of monocyte chemoattractant protein-1 (MCP-1) and transforming growth factor β1 (TGFβ1) in kidney stone formation. Recently, a genome-wide analysis of genes related to kidney stone formation and eliminiation in mice indicated that MCP-1 and TGFβ1 are involved in nephrolithiasis. In...

Fine structure of calcium oxalate monohydrate renal calculi.

Fine structure, location and size of the core of 12 calcium oxalate monohydrate (COM) papillar calculi from different 'idiopathic' stone-formers were studied by an optical and scanning electron microscope equipped with the EDAX analytical device. Each individual core exhibited a unique overall structure composed of loosely arranged twined and intergrown crystals of plate-like and/or columnar sh...

Regulation of renal epithelial cell affinity for calcium oxalate monohydrate crystals.

The binding and internalization of calcium oxalate monohydrate (COM) crystals by tubular epithelial cells may be a critical step leading to kidney stone formation. Exposure of MDCK cells to arachidonic acid (AA) for 3 days, but not oleic or linoleic acid, decreased COM crystal adhesion by 55%. Exogenous prostaglandin PGE(1) or PGE(2) decreased crystal binding 96% within 8 h, as did other agents...